Aberration measurement and correction on a large field of view in fluorescence microscopy
Tommaso Furieri, Daniele Ancora, Gianmaria Calisesi, Stefano Morara, Andrea Bassi, Stefano Bonora
The aberrations induced by the sample and/or by the sample holder limit the
resolution of optical microscopes. Wavefront correction can be achieved using a
deformable mirror with wavefront sensorless optimization algorithms but,
despite the complexity of these systems, the level of correction is often
limited to a small area in the field of view of the microscope. In this work,
we present a plug and play module for aberration measurement and correction.
The wavefront correction is performed through direct wavefront reconstruction
using the spinning-pupil aberration measurement and controlling a deformable
lens in closed loop. The lens corrects the aberrations in the center of the
field of view, leaving residual aberrations at the margins, that are removed by
anisoplanatic deconvolution. We present experimental results obtained in
fluorescence microscopy, with a wide field and a light sheet fluorescence
microscope. These results indicate that detection and correction over the full
field of view can be achieved with a compact transmissive module placed in the
detection path of the fluorescence microscope.